Journal: Journal of Clinical Microbiology
Article Title: Consequences of the Edge Effect in a Commercial Enzyme-Linked Immunosorbent Assay for the Diagnosis of Lyme Neuroborreliosis
doi: 10.1128/JCM.03280-20
Figure Lengend Snippet: Scatter dot plots of the optical density (OD) values of the positive kit control in the three experiments. The horizontal lines indicate the median OD values, and the whiskers represent the interquartile ranges (IQR; first and third quartiles). The displayed P values are raw P values that were found to be significant after applying the Benjamini-Hochberg procedure to account for the multiple comparisons in this study. (A) Scatter dot plots of the OD values of the positive kit control of which a single dilution (1:231) was tested in 30 wells of 4 strips that were placed in the first 4 columns of a 96-well plate holder on an automated DS2 instrument (Dynex Technologies). (B) Like panel A, however, with an additional strip filled with distilled water placed in the fifth column of the 96-well plate holder. (C) Scatter dot plots of the OD values of the positive kit control of which a single dilution (1:231) was tested in 48 wells in 6 strips that were placed in the first 6 columns of a 96-well plate holder on an automated BEP 2000 system (Siemens Healthcare Diagnostics).
Article Snippet: In this study, we showed that the impact of the edge effect on CSF-serum pairs could be reduced by some minor adaptations to the standard protocol of the manufacturer , which, in this case, only applies for testing on the Dynex DS2 automated ELISA instrument (Dynex Technologies).
Techniques: Control, Stripping Membranes